AKUdatabase

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Expression and functional characterization of AKU alleles in E. coli.
(from: José M. Rodríguez et al., 2000)

 
TABLE1. Enzyme activities of mutant HGO proteins


HGO protein


Specific Activity (nmol/min mg)


%WT
WT 20932 ± 1394

100
E42A 5972 ± 787

29
W60G 13,2

0.06
Y62C 4708 ± 834

22.5
H80Q 19275

95
W97G 22.5

0.1
A122D 6842 ± 1760

33.5
D153G 6408 ± 1966

32.7
G161R 204 ± 80

1
S189I 700 ± 43

3.4
I216T 1.4

0
R225H 23 ± 9

0.1
F227S 20.45

0.1
P230S 850 ± 306

4
P230T 210

1
D291E 0

0
V300G 400

1.9
R330S 93

0.4
M368V 7715 ± 1749

37
H371R 0

0

 

 
TABLE 2. Steady-state kinetic parameters of AKU HGO proteins showing significant activity


Mutant protein change


Vmax
(nmol/min mg)


Km (mM)


Vmax/Km


% wt

wt

22300

6,2

3597

100,0

E42A

6692

25,4

263

7,3

Y62C

4896

6

816

22,7

A122D

8382

9,2

911

25,3

D153G

6309

7,1

889

24,7

M368V

7400

14,4

514

14,3